Transgelin antibody (full length)

Catalog No. ABIN3027031

The Mouse Monoclonal anti-Transgelin antibody (Clone TAGLN-247) (ABIN3027031) specifically detects Transgelin in IHC (p). The antibody is reactive with Human samples.

Quick Overview for Transgelin antibody (full length) (ABIN3027031)

Target: Transgelin (TAGLN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transgelin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: TAGLN-247

Product Details anti-Transgelin Antibody

Binding Specificity: full length

Purpose: SM22 Antibody (azide and preservative free)

Characteristics: This mAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the SM22 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the SM22 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the SM22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: SM22

Background: This mAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

UniProt: Q01995