His Tag antibody

Catalog No. ABIN3028711

This Rabbit Polyclonal antibody specifically detects His Tag in WB and ELISA. It exhibits reactivity toward .

Quick Overview for His Tag antibody (ABIN3028711)

Target: His Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Purification: Purified

Immunogen: Poly-His peptide was used as the immungen for the His Tag antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.