XBP1 antibody (C-Term)

Catalog No. ABIN302900

The Rabbit Polyclonal anti-XBP1 antibody is suitable to detect XBP1 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for XBP1 antibody (C-Term) (ABIN302900)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XBP1 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to a 17 amino acid peptide from near the carboxy terminus of XBP-1.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a 17 amino acid peptide from near the Carboxy terminus of XBP-1.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western Blot: 1-2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline, containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at-20 °C for longer.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1 / TREB5

Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2

Gene ID: 7494

NCBI Accession: NP_001073007

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response