Src antibody (AA 614-826)

Catalog No. ABIN3029555

The Rabbit Polyclonal anti-Src antibody has been validated for WB. It is suitable to detect Src in samples from Human.

Quick Overview for Src antibody (AA 614-826) (ABIN3029555)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Src Antibody

Binding Specificity: AA 614-826

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 614-826) was used as the immunogen for this Src antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Src antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: Src

Background: The nuclear receptor coactivator 1 (NCOA1), also known as Src1, is a transcriptional coregulatory protein that contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. Src is recruited to DNA promotion sites by ligand-activated nuclear receptors. It in turn acylates histones, which makes downsteam DNA more accessible to transcription. Hence, it assists nuclear receptors in the upregulation of DNA expression. It has been found that the protein can enhance the transcriptional activity of ligand-bound PGR but does not alter the basal activity of the target promoter. It also enhances estrogen receptor, glucocorticoid receptor, thyroid hormone receptor, and retinoid X receptor transcriptional activities through their cognate DNA response elements in the presence of hormone. Additionally, Src may play a role as a bridging molecule between nuclear hormone receptors and general transcription factors.

Gene ID: 8648

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling