ANO1 antibody (C-Term)

Catalog No. ABIN3029980

This Rabbit Polyclonal antibody specifically detects ANO1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ANO1 antibody (C-Term) (ABIN3029980)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ANO1 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human ANO1/TMEM16A/DOG1 (KVLMVELFMREEQDKQQ) was used as the immunogen for this DOG1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the DOG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the DOG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: DOG1 (TMEM16A)

Background: Anoctamin-1 (ANO1), also known as oral cancer overexpressed 2 (ORAOV2), Tumor-amplified and overexpressed sequence 2 (TMEM16A), and Discovered on gastrointestinal stromal tumors protein 1 (DOG1) is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is though to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1/DOG1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.

UniProt: Q5XXA6