BUB3 antibody (Middle Region)

Catalog No. ABIN3030213

The Rabbit Polyclonal anti-BUB3 antibody (ABIN3030213) specifically detects BUB3 in WB, IHC (p), ICC and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BUB3 antibody (Middle Region) (ABIN3030213)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BUB3 Antibody

Binding Specificity: Middle Region

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human Bub3 (DHQLKMHDLNTDQEN) was used as the immunogen for this Bub3 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Bub3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Bub3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: Bub3

Background: Budding uninhibited by benzimidazoles 3 is a protein that in humans is encoded by the BUB3 gene. It is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC), though Bub3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, it delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. It also acts as a regulator in that it affects binding of Mad3 to Mad2.

UniProt: O43684

Pathways: Maintenance of Protein Location