Caspase 3 antibody (AA 67-175)

Catalog No. ABIN3030302

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for WB, IHC (p) and FACS. It is suitable to detect Caspase 3 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 3 antibody (AA 67-175) (ABIN3030302)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 67-175

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 67-175) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the large subunit.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: Caspase-3 is an enzyme which interacts with Survivin, XIAP, CFLAR, CASP8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. They exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. CASP3 is the predominant cysteine-aspartic acid protease involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. And protein activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the proteins influence on bone mineral density should be considered in any in vivo application of Caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Gene ID: 836

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation