beta Catenin antibody (N-Term)

Catalog No. ABIN3030327

This anti-beta Catenin antibody is a Rabbit Polyclonal antibody detecting beta Catenin in WB, IHC (p), ICC and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for beta Catenin antibody (N-Term) (ABIN3030327)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-beta Catenin Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: A synthetic peptide corresponding to the N-terminus of the human protein (MATQADLMELDMAMEPDRK) was used as the immunogen for this b-Catenin antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the b-Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the b-Catenin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: B-Catenin

Background: Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin, which can bind to beta-catenin and can also bind actin, and beta-catenin. b-Catenin binds the cytoplasmic domain of some cadherins as an adherens junction protein. In normal tissues, b-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy, HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

UniProt: P35222

Pathways: Peptide Hormone Metabolism