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CD34 antibody (AA 151-385)

The Rabbit Polyclonal anti-CD34 antibody has been validated for FACS, WB and IHC (p). It is suitable to detect CD34 in samples from Human, Rat and Mouse.
Catalog No. ABIN3030386

Quick Overview for CD34 antibody (AA 151-385) (ABIN3030386)

Target

See all CD34 Antibodies
CD34

Reactivity

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Human, Rat, Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This CD34 antibody is un-conjugated

Application

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    AA 151-385

    Purification

    Antigen affinity

    Immunogen

    Human partial recombinant protein (AA 151-385) was used as the immunogen for this CD34 antibody.

    Isotype

    IgG
  • Application Notes

    The stated application concentrations are suggested starting amounts. Titration of the CD34 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

    Restrictions

    For Research Use only
  • Buffer

    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

    Storage

    -20 °C

    Storage Comment

    After reconstitution, the CD34 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target

    CD34

    Alternative Name

    CD34

    Background

    CD34 is a monomeric cell surface antigen with a glycosylated molecular mass of approximately 110 KD. It is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene is located on human chromosome 1. It plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

    Gene ID

    947
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