CD44 antibody (C-Term)

Catalog No. ABIN3030402

This anti-CD44 antibody is a Rabbit Polyclonal antibody detecting CD44 in WB, IHC (p), IHC (fro) and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for CD44 antibody (C-Term) (ABIN3030402)

Target: CD44

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-CD44 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity purified

Immunogen: An amino acid sequence from the C-terminus of human CD44 (DETRNLQNVDMKIGV) was used as the immunogen for this CD44 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CD44 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CD44 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: The CD44 gene, which is a transmembrane protein, is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. The gene, which contains 19 exons spanning some 50 kb of genomic DNA, is a widely expressed integral membrane protein that acts as a receptor for hyaluronan(HA) and is important to cell-extracellular matrix interaction. CD44 binding with HA can play an important role in cellular aggregation and tumor cell growth. CD44 is necessary for limb development and functions in a novel growth factor presentation mechanism. A specific CD44 splice variant is crucial for the proliferation of these mesenchymal cells. CD44 glycoproteins are involved in leukocyte extravasation but also in the regulation of growth factor activation, stability, and signaling. Moreover, it plays a pivotal role in arteriogenesis.

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling