CDC25B antibody (AA 541-559)

Catalog No. ABIN3030454

This Rabbit Polyclonal antibody specifically detects CDC25B in WB and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for CDC25B antibody (AA 541-559) (ABIN3030454)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC25B Antibody

Binding Specificity: AA 541-559

Purification: Antigen affinity

Immunogen: Amino acids 541-559 (DYRPMNHEAFKDELKTFRL-human) were used as the immunogen for this Cdc25B antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Cdc25B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Cdc25B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: Cdc25B

Background: Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human< a href=../search_result.php?search_txt=cdc25>CDC25genes code for the A, B, and C forms of CDC25.< a href=../search_result.php?search_txt=cdc25b>CDC25Bis mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and< a href=../search_result.php?search_txt=cdc25c>CDC25Cat serine-216, phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy