CRTC1 antibody

Catalog No. ABIN303081

This anti-CRTC1 antibody is a Rabbit Polyclonal antibody detecting CRTC1 in WB, IF, IHC (p) and EIA. Suitable for Human, Mouse, Rat, Cow and Zebrafish (Danio rerio).

Quick Overview for CRTC1 antibody (ABIN303081)

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CRTC1 Antibody

Specificity: This antibody recognizes amino acids 19-34 of Human MECT1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Zebrafish, Bovine.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide from Human CRTC1 / MECT1.

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on paraffin sections: 1: 500. Western Blot: 1: 500 - 1: 1000. ELISA: 1: 10000 - 1: 40000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CRTC1

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Alternative Name: CRTC1 / MECT1

Background: MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1, Transducer of regulated cAMP response element-binding protein 1, TORC1, and Transducer of CREB protein 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11,19) (q21,p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.Synonyms: CREB-regulated transcription coactivator 1, KIAA0616, Mucoepidermoid carcinoma translocated protein 1, TORC1, Transducer of regulated cAMP response element-binding protein 1, WAMTP1

Gene ID: 23373

NCBI Accession: NP_001091952

UniProt: Q6UUV9