Flotillin 1 antibody (Middle Region)

Catalog No. ABIN3030972

This anti-Flotillin 1 antibody is a Rabbit Polyclonal antibody detecting Flotillin 1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for Flotillin 1 antibody (Middle Region) (ABIN3030972)

Target: Flotillin 1 (FLOT1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Flotillin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Flotillin 1 Antibody

Binding Specificity: Middle Region

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human FLOT1 (KKAAYDIEVNTRRAQA) was used as the immunogen for this Flotillin 1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Flotillin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Flotillin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Flotillin 1

Target: Flotillin 1 (FLOT1)

Alternative Name: Flotillin 1

Background: Flotillin 1 is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with Flotillin1 in detergent-insoluble membrane fractions. Flotillin1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.

UniProt: O75955