GJB2 antibody (N-Term)

Catalog No. ABIN3031025

The Rabbit Polyclonal anti-GJB2 antibody has been validated for WB. It is suitable to detect GJB2 in samples from Human, Mouse and Rat.

Quick Overview for GJB2 antibody (N-Term) (ABIN3031025)

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GJB2 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human GJB2 (MDWGTLQTILGGVNKH) was used as the immunogen for this GJB2 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the GJB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the GJB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2

Background: Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5 % identity with rat Cx26. connexin 26(GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene(GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization