Gelsolin antibody (AA 763-775)

Catalog No. ABIN3031036

Product Details anti-Gelsolin Antibody

Target: Gelsolin (GSN)

Binding Specificity: AA 763-775

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: Amino acids 763-775 (WDDDYWSVDPLDR-human) were used as the immunogen for this Gelsolin antibody (100% rat homology).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Gelsolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Gelsolin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: Gelsolin (GSN Products)

Background: Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. It is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor, it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. It may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of Gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

UniProt: P06396

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy