ITGAL antibody (AA 404-769)

Catalog No. ABIN3031369

This Rabbit Polyclonal antibody specifically detects ITGAL in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ITGAL antibody (AA 404-769) (ABIN3031369)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ITGAL Antibody

Binding Specificity: AA 404-769

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 404-769) was used as the immunogen for this LFA-1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the LFA-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IF/ICC: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the LFA-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: LFA-1

Background: Lymphocyte function associated antigen 1, also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. It is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated LFA-1/CD18. The expression of LFA-1 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of LFA-1 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although the protein is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.

Gene ID: 3689

Pathways: Activated T Cell Proliferation, Integrin Complex