IL-3 antibody (AA 20-152)

Catalog No. ABIN3031475

The Rabbit Polyclonal anti-IL-3 antibody has been validated for WB, IHC (p) and ELISA (Standard). It is suitable to detect IL-3 in samples from Human.

Quick Overview for IL-3 antibody (AA 20-152) (ABIN3031475)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA (Standard)

Product Details anti-IL-3 Antibody

Binding Specificity: AA 20-152

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 20-152) was used as the immunogen for this human IL3 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the human IL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot (recombinant protein): 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,ELISA: 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the human IL3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3

Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy