KRT8 antibody (N-Term)

Catalog No. ABIN3031541

The Rabbit Polyclonal anti-KRT8 antibody has been validated for WB and IHC (p). It is suitable to detect KRT8 in samples from Human, Rat and Mouse.

Quick Overview for KRT8 antibody (N-Term) (ABIN3031541)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KRT8 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human Cytokeratin 8 (QQQKTARSNMDNMFESYIN) was used as the immunogen for this Cytokeratin 8 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Cytokeratin 8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Cytokeratin 8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Cytokeratin 8(CK8), also known as Keratin 8(K8), is a type II keratin. Among the various intermediate filament(IF) proteins, cytokeratin 8(CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney. Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy. He et al.(2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling. The functional significance of this phosphorylation could relate to regulation of JNK signaling and/or regulation of keratin dynamics.

UniProt: P05787