ERK1/2 antibody (N-Term)

Catalog No. ABIN3031825

This Rabbit Polyclonal antibody specifically detects ERK1/2 in WB, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ERK1/2 antibody (N-Term) (ABIN3031825)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ERK1/2 Antibody

Binding Specificity: N-Term

Purpose: MAPK1/3 Antibody / ERK1/2

Sequence: ARVADPDHDH TGFL

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human MAPK1/3 (ARVADPDHDHTGFL) was used as the immunogen for this MAPK1/3 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the MAPK1/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MAPK1/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: MAPK1/3

Background: MAPK1 (ERK2) shares high homology with MAPK3 (ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases (also known as ERKs) have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.

UniProt: P28482