MAG antibody (AA 114-132)

Catalog No. ABIN3031888

Product Details anti-MAG Antibody

Target: MAG (Myelin Associated Glycoprotein (MAG))

Binding Specificity: AA 114-132

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: Amino acids 114-132 (KYYFRGDLGGYNQYTFSEH) were used as the immunogen for this MAG antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the MAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. MAG is seen in western blotting from 60~100KD depending on the level of glycosylation.\. Western blot: 0.5-1 μg/mL (1),IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the MAG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: MAG (MAG Products)

Synonyms: GMA antibody, S-MAG antibody, SIGLEC-4A antibody, SIGLEC4A antibody, siglec-4 antibody, MAG antibody, Gma antibody, siglec-4a antibody, myelin associated glycoprotein antibody, myelin-associated glycoprotein antibody, MAG antibody, mag antibody, Mag antibody

Background: Myelin-associated glycoprotein, also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. Resistant embryonic neurons were rendered sensitive by expression of NgR. The glycoprotein binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with the glycoprotein prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway