NOX5 antibody (C-Term)

Catalog No. ABIN3031936

This Rabbit Polyclonal antibody specifically detects NOX5 in WB, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NOX5 antibody (C-Term) (ABIN3031936)

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NOX5 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human NADPH Oxidase 5 (KVLKGHCEKFGFRFFQENF) was used as the immunogen for this NOX5 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the NOX5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the NOX5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for NOX5

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Alternative Name: NOX5

Background: NADPH Oxidase 5, also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. It is a NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.

UniProt: Q96PH1

Pathways: Proton Transport