NOX5 antibody (Internal Region)

Catalog No. ABIN571285

Product Details anti-NOX5 Antibody

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: NOX5

Sequence: EWHPFTISSA PEQKD

Specificity: This antibody is expected to recognize all reported isoforms (NP_078781.3, NP_001171708.1, NP_001171709.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-EWHPFTISSAPEQKD

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in Human Testis lysates (calculated MW of 86.4 kDa according to NP_078781.3). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOX5

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Alternative Name: NOX5 (NOX5 Products)

Synonyms: NADPH oxidase 5 antibody, NOX5 antibody

Background: NOX5, NADPH oxidase, EF-hand calcium binding domain 5, MGC149776, MGC149777, NADPH oxidase 5, OTTHUMP00000164443, OTTHUMP00000164444

Gene ID: 79400

NCBI Accession: NP_078781, NP_001171708, NP_001171709

Pathways: Proton Transport