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HMGN1 antibody (pSer20, pSer24)

HMGN1 Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Frog, Monkey, Pig EIA, IF, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN303233
$633.60
Plus shipping costs $45.00
50 μg
Shipping to: United States
Available
  • Target See all HMGN1 Antibodies
    HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
    Binding Specificity
    • 18
    • 16
    • 15
    • 15
    • 9
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 19-28, pSer20, pSer24
    Reactivity
    • 73
    • 17
    • 14
    • 8
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Cow, Dog, Frog, Monkey, Pig
    Host
    • 76
    • 1
    Rabbit
    Clonality
    • 76
    • 1
    Polyclonal
    Conjugate
    • 35
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This HMGN1 antibody is un-conjugated
    Application
    • 40
    • 32
    • 29
    • 26
    • 26
    • 15
    • 11
    • 7
    • 4
    • 2
    • 1
    • 1
    Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purification
    Immunoaffinity Chromatography.
    Immunogen
    Synthetic peptide corresponding to Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24.
  • Application Notes
    Immunohistochemistry on Paraffin Sections (20 μg/mL). Immunofluorescence. Western Blot(1/500-1/2000). ELISA (1/5000-1/25000).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.
  • Target
    HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
    Alternative Name
    HMGN1 (HMGN1 Products)
    Synonyms
    HMG14 antibody, hmg14 antibody, hmgn1 antibody, hmgn1b antibody, HMG-14 antibody, Hmg14 antibody, high mobility group nucleosome binding domain 1 antibody, high mobility group nucleosome binding domain 1 S homeolog antibody, high mobility group nucleosomal binding domain 1 antibody, non-histone chromosomal protein HMG-14 antibody, HMGN1 antibody, hmgn1.S antibody, hmgn1 antibody, Hmgn1 antibody, LOC785479 antibody
    Background
    HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box," that of the HMGN family, the "nucleosomal binding domain," and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."Synonyms: HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1, Non-histone chromosomal protein HMG-14
    Gene ID
    3150
    NCBI Accession
    NP_004956
    UniProt
    P05114
    Pathways
    Chromatin Binding
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