HMGN1 antibody (Middle Region)

Catalog No. ABIN2780630

Cited in 1 publication. The Rabbit Polyclonal anti-HMGN1 antibody (ABIN2780630) specifically detects HMGN1 in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Pig, Dog, Cow, Guinea Pig and Rabbit samples.

Quick Overview for HMGN1 antibody (Middle Region) (ABIN2780630)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivity: Human, Mouse, Rat, Horse, Pig, Dog, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HMGN1 Antibody

Binding Specificity: Middle Region

Purpose: HMGN1 Antibody - middle region

Sequence: QTKGKRGAKG KQAEVANQET KEDLPAENGE TKTEESPASD EAGEKEAKSD

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 93%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against HMGN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HMGN1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38532-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGN1 antibody (ABIN2780630)

Lane, Chapuy, Lin, Tivey, Li, Townsend, van Bodegom, Day, Wu, Liu, Yoda, Alexe, Schinzel, Sullivan, Malinge, Taylor, Stegmaier, Jaffe, Bustin, te Kronnie, Izraeli, Harris, Stevenson, Neuberg et al.: "Triplication of a 21q22 region contributes to B cell transformation through HMGN1 overexpression and loss of histone H3 Lys27 trimethylation. ..." in: Nature genetics, Vol. 46, Issue 6, pp. 618-23, (2014) (PubMed).

Target Details for HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Alternative Name: HMGN1

Background:

Background Information: Chromosomal protein HMG14 (HMGN1) and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: High mobility group nucleosome binding domain 1

Alternative Symbols: HMG14

Protein Name: High-mobility group nucleosome binding domain 1 EMBL AAH70154.1

Molecular Weight: 11kDa

Gene ID: 3150

NCBI Accession: NP_004956

UniProt: Q6NSG7

Pathways: Chromatin Binding