SMAD1 antibody (C-Term)

Catalog No. ABIN3032612

The Rabbit Polyclonal anti-SMAD1 antibody has been validated for WB, IHC (p) and ICC. It is suitable to detect SMAD1 in samples from Human, Mouse and Rat.

Quick Overview for SMAD1 antibody (C-Term) (ABIN3032612)

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SMAD1 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of mouse SMAD family member 1 (AEISRGDVQAVAYEE) was used as the immunogen for this Smad1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the SMAD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1

Background: Mothers against decapentaplegic homolog 1, also known as SMAD family member 1, is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic(Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.

UniProt: P70340

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development