NOTCH2 antibody (cleaved, Intracellular, N-Term)

Catalog No. ABIN303375

The Rabbit Polyclonal anti-NOTCH2 antibody (ABIN303375) specifically detects NOTCH2 in IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for NOTCH2 antibody (cleaved, Intracellular, N-Term) (ABIN303375)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-NOTCH2 Antibody

Binding Specificity: cleaved, Intracellular, N-Term

Specificity: This antibody recognises Notch Homolog 2 (NOTCH2).

Purification: Sterile filtration.

Immunogen: Synthetic Peptide corresponding to Amino acid residues of human Notch 2 located near the N-terminal sequence of the Cleaved N Intracellular Domain (NICD).

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (1/500). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at-20 °C prior to opening. Aliquot contents and freeze at-20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature.

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: NOTCH2

Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.Synonyms: Neurogenic locus notch homolog protein 2, Notch 2, hN2

Gene ID: 4853

NCBI Accession: NP_001186930

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance