SLIT1 antibody (AA 487-504)

Catalog No. ABIN303378

The Rabbit Polyclonal anti-SLIT1 antibody (ABIN303378) specifically detects SLIT1 in WB, IHC (p), IF and EIA. The antibody is reactive with Human, Mouse, Chicken and Frog samples.

Quick Overview for SLIT1 antibody (AA 487-504) (ABIN303378)

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Reactivity: Human, Mouse, Chicken, Frog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-SLIT1 Antibody

Binding Specificity: AA 487-504

Specificity: This antibody recognises Slit Homolog 1 (SLIT1).

Cross-Reactivity (Details): Species reactivity (expected):Chicken, Frog.
Species reactivity (tested):Human and Mouse.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic Peptide corresponding to aa 487-504 of Mouse SLIT-1 protein.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunofluorescence. Western Blot (1/500-1/2000). ELISA (1/10000-1/50000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Target Details for SLIT1

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Alternative Name: SLIT1

Background: SLIT-1 (also known as KIAA0813, MEGF4, multiple epidermal growth factor-like domains 4 and Slit homolog 1 protein) is a Slit protein. This protein is a ligand for the Roundabout (Robo) receptors and acts as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. In situ hybridization studies indicated that the rat SLIT-1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. This data suggests that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Alternative splicing isoforms have been identified for Slit1 protein.Synonyms: KIAA0813, MEGF4, Multiple epidermal growth factor-like domains protein 4, SLIL1, SLIT-1, Slit homolog 1 protein

Gene ID: 6585

NCBI Accession: NP_003052

UniProt: O75093

Pathways: Regulation of Cell Size