HMGB3 antibody (N-Term)

Catalog No. ABIN3042447

This anti-HMGB3 antibody is a Rabbit Polyclonal antibody detecting HMGB3 in WB, IHC, ICC, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for HMGB3 antibody (N-Term) (ABIN3042447)

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HMGB3 Antibody

Binding Specificity: AA 62-95, N-Term

Purpose: Anti-HMG4/HMGB3 Antibody Picoband®

Sequence: EMAKADKVRY DREMKDYGPA KGGKKKKDPN APKR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HMG4/HMGB3 Antibody Picoband® (ABIN3042447). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: HMGB3 high-mobility group box 3". 2. Davis DL, Burch JB (1992). "Isolation of a chicken HMG2 cDNA clone and evidence for an HMG2-specific 3'-untranslated region.". Gene 113 (2): 251-6. 3. Vaccari T, Beltrame M, Ferrari S, Bianchi ME (Aug 1998). "Hmg4, a new member of the Hmg1/2 gene family".Genomics 49 (2): 247-52.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Alternative Name: HMGB3

Background:

Synonyms: High mobility group protein B3,High mobility group protein 2a,HMG-2a,High mobility group protein 4,HMG-4,HMGB3,HMG2A, HMG4,

Tissue Specificity: Expressed predominantly in placenta.

Background: High-mobility group protein B, also known as HMG4, is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 23 kDa

Gene ID: 3149

UniProt: O15347