HRG antibody (AA 19-200)

Catalog No. ABIN3042459

Product Details anti-HRG Antibody

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Binding Specificity: AA 19-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Histidine-rich glycoprotein(HRG) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Histidine-rich glycoprotein(HRG) detection. Tested with WB, IHC-P in Human.
Gene Name: histidine-rich glycoprotein
Protein Name: Histidine-rich glycoprotein

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HRG recombinant protein (Position: V19-V200). Human HRG shares 71% and 69% amino acid (aa) sequences identity with mouse and rat HRG, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for HRG is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HRG

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Alternative Name: HRG (HRG Products)

Synonyms: AI265597 antibody, AW413091 antibody, D16jh2 antibody, D18020 antibody, Hprg antibody, Hrgp antibody, HPRG antibody, HRGP antibody, THPH11 antibody, HRG1 antibody, histidine rich glycoprotein antibody, histidine-rich glycoprotein antibody, HRG antibody, LOAG_12427 antibody, LOC100597044 antibody, Hrg antibody

Background: Histidine-rich glycoprotein (HRG) is a protein that in humans is encoded by the HRG gene. It is mapped to 3q27.3. HRG contains two cystatin-like domains and is located in plasma and platelets. The protein can bind heme, dyes and divalent metal ions. It also can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. HRG is thought to have multiple roles as a protein in the human blood, including roles in immunity, angiogenesis and coagulation.

Synonyms: DKFZp779H1622 antibody|Histidine proline rich glycoprotein antibody|Histidine rich glycoprotein antibody|HPRG antibody|HRGP antibody|Thrombophilia due to elevated HRG antibody

Gene ID: 3273

UniProt: P04196