HSF4 antibody (N-Term)

Catalog No. ABIN3042461

The Rabbit Polyclonal anti-HSF4 antibody has been validated for WB. It is suitable to detect HSF4 in samples from Human.

Quick Overview for HSF4 antibody (N-Term) (ABIN3042461)

Target: HSF4 (Heat Shock Transcription Factor 4 (HSF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSF4 Antibody

Binding Specificity: AA 149-185, N-Term

Purpose: Anti-HSF4 Antibody Picoband®

Sequence: VQESTEARLR ELRQQNEILW REVVTLRQSH GQQHRVI

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HSF4 Antibody Picoband® (ABIN3042461). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSF4, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Bu L, Jin Y, Shi Y, Chu R, Ban A, Eiberg H, Andres L, Jiang H, Zheng G, Qian M, Cui B, Xia Y, Liu J, Hu L, Zhao G, Hayden MR, Kong X (Jun 2002). "Mutant DNA-binding domain of HSF4 is associated with autosomal dominant lamellar and Marner cataract". Nat Genet 31 (3): 276-8. 2. Nakai A, Tanabe M, Kawazoe Y, Inazawa J, Morimoto RI, Nagata K (Jan 1997). "HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator". Mol Cell Biol 17 (1): 469-81. 3. Tanabe M, Sasai N, Nagata K, Liu XD, Liu PC, Thiele DJ, Nakai A (Nov 1999). "The mammalian HSF4 gene generates both an activator and a repressor of heat shock genes by alternative splicing". J Biol Chem 274(39): 27845-56.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HSF4

Target: HSF4 (Heat Shock Transcription Factor 4 (HSF4))

Alternative Name: HSF4

Background:

Synonyms: Heat shock factor protein 4,HSF 4,hHSF4,Heat shock transcription factor 4,HSTF 4,HSF4,

Tissue Specificity: Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .

Background: Heat shock factor protein 4 is a protein that in humans is encoded by the HSF4 gene. It is mapped to 16q21. Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 53 kDa

Gene ID: 3299

Pathways: MAPK Signaling