IRF2 antibody (C-Term)

Catalog No. ABIN3042468

This anti-IRF2 antibody is a Rabbit Polyclonal antibody detecting IRF2 in WB. Suitable for Human and Rat.

Quick Overview for IRF2 antibody (C-Term) (ABIN3042468)

Target: IRF2 (Interferon Regulatory Factor 2 (IRF2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IRF2 Antibody

Binding Specificity: AA 317-348, C-Term

Purpose: Anti-IRF2 Antibody Picoband®

Sequence: MTPASSSSRP DRETRASVIK KTSDITQARV KS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IRF2 Antibody Picoband® (ABIN3042468). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IRF2, different from the related mouse sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Harada, H., Takahashi, E.-I., Itoh, S., Harada, K., Hori, T.-A., Taniguchi, T. Structure and regulation of the human interferon regulatory factor 1 (IRF-1) and IRF-2 genes: implications for a gene network in the interferon system. Molec. Cell. Biol. 14: 1500-1509, 1994. 2. Nishio, Y., Noguchi, E., Ito, S., Ichikawa, E., Umebayashi, Y., Otsuka, F., Arinami, T. Mutation and association analysis of the interferon regulatory factor 2 gene (IRF2) with atopic dermatitis. J. Hum. Genet. 46: 664-667, 2001. 3. Taki, S., Nakajima, S., Ichikawa, E., Saito, T., Hida, S. IFN regulatory factor-2 deficiency revealed a novel checkpoint critical for the generation of peripheral NK cells. J. Immun. 174: 6005-6012, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IRF2

Target: IRF2 (Interferon Regulatory Factor 2 (IRF2))

Alternative Name: IRF2

Background:

Synonyms: Interferon regulatory factor 2,IRF-2,IRF2,

Tissue Specificity: Expressed throughout the epithelium of the colon. Also expressed in lamina propria. .

Background: IRF2 (interferon regulatory factor 2) is a member of the interferon regulatory transcription factor (IRF) family. The IRF2 gene is mapped on 4q35.1. When the IRF2 gene was overexpressed in NIH 3T3 cells, the cells became transformed and displayed enhanced tumorigenicity in nude mice. One IRF binding site was found within the IRF2 promoter, and expression of the IRF2 gene was affected by both transient and stable IRF1 expression. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. Irf2 was required to prevent NK-cell apoptosis and keep immature NK cells alive, thus promoting NK-cell maturation and their supply to peripheral blood.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 50 kDa

Gene ID: 3660

UniProt: P14316