KAT2A antibody (N-Term)

Catalog No. ABIN3042472

This Rabbit Polyclonal antibody specifically detects KAT2A in WB. It exhibits reactivity toward Human.

Quick Overview for KAT2A antibody (N-Term) (ABIN3042472)

Target: KAT2A (K(lysine) Acetyltransferase 2A (KAT2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAT2A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KAT2A Antibody

Binding Specificity: AA 75-106, N-Term

Purpose: Anti-KAT2A/GCN5 Antibody Picoband®

Sequence: DPARPGLSQQ QRASQRKAQV RGLPRAKKLE KL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-KAT2A/GCN5 Antibody Picoband® (ABIN3042472). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KAT2A/GCN5, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: GCN5L2 GCN5 general control of amino-acid synthesis 5-like 2 (yeast)". 2. Candau R, Moore PA, Wang L, Barlev N, Ying CY, Rosen CA, Berger SL (February 1996). "Identification of human proteins functionally conserved with the yeast putative adaptors ADA2 and GCN5". Mol Cell Biol 16 (2): 593-602. 3. Wang L, Mizzen C, Ying C, Candau R, Barlev N, Brownell J, Allis CD, Berger SL (January 1997). "Histone acetyltransferase activity is conserved between yeast and human GCN5 and is required for complementation of growth and transcriptional activation". Mol. Cell. Biol. 17 (1): 519-27.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KAT2A

Target: KAT2A (K(lysine) Acetyltransferase 2A (KAT2A))

Alternative Name: KAT2A

Background:

Synonyms: Histone acetyltransferase KAT2A,2.3.1.48,General control of amino acid synthesis protein 5-like 2,Histone acetyltransferase GCN5,HsGCN5,Lysine acetyltransferase 2A,STAF97,KAT2A,GCN5, GCN5L2, HGCN5,

Tissue Specificity: Expressed in all tissues tested, with most abundant expression in ovary.

Background: Histone acetyltransferase KAT2A?is an?enzyme?that in humans is encoded by the?KAT2A?gene. It is mapped to 17q21. KAT2A, or GCN5, GCN5L2, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. The GCN5 protein, a regulator of transcription activation in yeast, promotes maximal levels of transcription by 2 transcriptional activators, GCN4 and the HAP2-HAP3-HAP4 complex.?The GCN4 protein activates transcription of a large number of amino acid biosynthetic genes under limiting amino acid conditions and the HAP2-HAP3-HAP4 complex is thought to mediate transcription of genes involved in respiratory functions.?GCN5 also functions as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT-independent manner.

Molecular Weight: 94 kDa

Gene ID: 2648

UniProt: Q92830

Pathways: Chromatin Binding, Tube Formation