S100A8 antibody (AA 2-89)

Catalog No. ABIN3042481

This Rabbit Polyclonal antibody specifically detects S100A8 in WB, IHC, ICC, IF and FACS. It exhibits reactivity toward Mouse and Rat.

Quick Overview for S100A8 antibody (AA 2-89) (ABIN3042481)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-S100A8 Antibody

Binding Specificity: AA 2-89

Purpose: Anti-MRP8/S100A8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with recombinant human S100A8 is observed.

Characteristics: Anti-MRP8/A8 Antibody Picoband® (ABIN3042481). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
1. "Entrez Gene: S100A8 S100 calcium binding protein A8". 2. Kerkhoff C, Klempt M, Sorg C (1999). "Novel insights into structure and function of MRP8 (S100A8) and MRP14 (S100A9).". Biochim. Biophys. Acta 1448 (2): 200-11. 3. Schäfer T, Sachse GE, Gassen HG (1991). "The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells.". Biol. Chem. Hoppe-Seyler 372 (1): 1-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100a8

Background:

Synonyms: Protein S100-A8,Calgranulin-A,Chemotactic cytokine CP-10,Leukocyte L1 complex light chain,Migration inhibitory factor-related protein 8,MRP-8,p8,Pro-inflammatory S100 cytokine,S100 calcium-binding protein A8,S100a8,Caga, Mrp8,

Tissue Specificity: Ubiquitously expressed, with highest levels in skeletal muscle, heart and thymus. Overexpressed in prostate tumor tissue. .

Background: S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Molecular Weight: 11 kDa

Gene ID: 20201

UniProt: P27005

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins