SOCS2 antibody (AA 96-198)

Catalog No. ABIN3042487

This Rabbit Polyclonal antibody specifically detects SOCS2 in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for SOCS2 antibody (AA 96-198) (ABIN3042487)

Target: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOCS2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SOCS2 Antibody

Binding Specificity: AA 96-198

Purpose: Anti-SOCS2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SOCS2 Antibody Picoband® (ABIN3042487). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SOCS2 recombinant protein (Position: R96-V198). Human SOCS2 shares 96.1% and 95.1% amino acid (aa) sequence identity with mouse and rat SOCS2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: SOCS2 suppressor of cytokine signaling 2". 2. Dey BR, Spence SL, Nissley P, Furlanetto RW (September 1998). "Interaction of human suppressor of cytokine signaling (SOCS)-2 with the insulin-like growth factor-I receptor". J. Biol. Chem. 273 (37): 24095-101. 3. Greenhalgh CJ, Bertolino P, Asa SL, Metcalf D, Corbin JE, Adams TE, Davey HW, Nicola NA, Hilton DJ, Alexander WS (June 2002). "Growth enhancement in suppressor of cytokine signaling 2 (SOCS-2)-deficient mice is dependent on signal transducer and activator of transcription 5b (STAT5b)". Mol. Endocrinol. 16 (6): 1394-406. 4. Masuhara M, Sakamoto H, Matsumoto A, Suzuki R, Yasukawa H, Mitsui K, Wakioka T, Tanimura S, Sasaki A, Misawa H, Yokouchi M, Ohtsubo M, Yoshimura A (Nov 1997). "Cloning and characterization of novel CIS family genes". Biochem Biophys Res Commun 239 (2): 439-46. 5. Minamoto S, Ikegame K, Ueno K, Narazaki M, Naka T, Yamamoto H, Matsumoto T, Saito H, Hosoe S, Kishimoto T (Sep 1997). "Cloning and functional analysis of new members of STAT induced STAT inhibitor (SSI) family: SSI-2 and SSI-3". Biochem Biophys Res Commun 237 (1): 79-83.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SOCS2

Target: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Alternative Name: SOCS2

Background:

Synonyms: Suppressor of cytokine signaling 2,SOCS-2,Cytokine-inducible SH2 protein 2,CIS-2,STAT-induced STAT inhibitor 2,SSI-2,SOCS2,CIS2, SSI2, STATI2,

Tissue Specificity: High expression in heart, placenta, lung, kidney and prostate.

Background: Suppressor of cytokine signaling 2 is a protein that in humans is encoded by the SOCS2 gene. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signalling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10 and interferon-gamma (IFN-gamma). The protein encoded by this gene is found to interact with the cytoplasmic domain of insulin-like growth factor 1 receptor (IGF1R), and thus is thought to be involved in the regulation of IGF1R mediated cell signaling. By cytogenetic and radiation hybrid mapping, Yandava et al. (1999) mapped the SOCS2 gene to chromosome 12q21.3-q23.

Sequence Similarities: Contains 1 SH2 domain.

Molecular Weight: 22 kDa

Gene ID: 8835

UniProt: O14508

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus