Mesothelin antibody (AA 308-576)

Catalog No. ABIN3042494

The Rabbit Polyclonal anti-Mesothelin antibody has been validated for IHC. It is suitable to detect Mesothelin in samples from Mouse.

Quick Overview for Mesothelin antibody (AA 308-576) (ABIN3042494)

Target: Mesothelin (MSLN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-Mesothelin Antibody

Binding Specificity: AA 308-576

Purpose: Anti-Mesothelin/MSLN Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Mesothelin/MSLN Antibody Picoband® (ABIN3042494). Tested in IHC applications. This antibody reacts with Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576). Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
1. Bera, T. K., Pastan, I. Mesothelin is not required for normal mouse development or reproduction. Molec. Cell. Biol. 20: 2902-2906, 2000. 2. Scholler, N., Fu, N., Yang, Y., Ye, Z., Goodman, G. E., Hellstrom, K. E., Hellstrom, I. Soluble member(s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma. Proc. Nat. Acad. Sci. 96: 11531-11536, 1999.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: Msln

Background:

Synonyms: Mesothelin,Pre-pro-megakaryocyte-potentiating factor,Megakaryocyte-potentiating factor,MPF,Mesothelin, cleaved form,Msln,Mes, Mpf,

Tissue Specificity: Highly expressed in lung and heart. Expressed at low levels in spleen, liver, kidney and testis. Present in lung (at protein level). .

Background: Mesothelin, also known as MSLN or MPF, is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Molecular Weight: 54 kDa

Gene ID: 56047

UniProt: Q61468

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process