PIN1 antibody (AA 1-163)

Catalog No. ABIN3042501

This anti-PIN1 antibody is a Rabbit Polyclonal antibody detecting PIN1 in WB and FACS. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for PIN1 antibody (AA 1-163) (ABIN3042501)

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIN1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PIN1 Antibody

Binding Specificity: AA 1-163

Purpose: Anti-Pin1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Pin1 Antibody Picoband® (ABIN3042501). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Pin1 recombinant protein (Position: M1-E163). Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Liou, Y.-C., Ryo, A., Huang, H.-K., Lu, P.-J., Bronson, R., Fujimori, F., Uchida, T., Hunter, T., Lu, K. P. Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes. Proc. Nat. Acad. Sci. 99: 1335-1340, 2002. 2. Lu, KP, Finn G, Lee TH, Nicholson LK (Oct 2007). "Prolyl cis-trans isomerization as a molecular timer".Nat. Chem. Biol. (United States) 3 (10): 619-29.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PIN1

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Alternative Name: PIN1

Background:

Synonyms: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1,5.2.1.8,Peptidyl-prolyl cis-trans isomerase Pin1,PPIase Pin1,Rotamase Pin1,PIN1,

Tissue Specificity: The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells. .

Background: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, also called DOD, is an enzyme that in humans is encoded by the PIN1 gene. It is mapped to 19p13.2. The enzyme binds to a subset of proteins and thus plays a role as a post phospho PIN1rol in regulating protein function. Studies have shown that the deregulation of PIN1 may play a pivotal role in various diseases. Notably, the up-regulation of PIN1 may be implicated in certain cancers, and the down-regulation of Pin1 may be implicated in Alzheimer's disease. Inhibitors of PIN1 may have therapeutic implications for cancer and immune disorders. PIN1 activity regulates the outcome of proline-ed kinase (e.g. MAPK, CDK or GSK3) signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell survival. PIN1 also has an essential role in maintaining cell proliferation and regulating cyclin D1 function.

Sequence Similarities: Contains 1 PpiC domain.

Molecular Weight: 18 kDa

Gene ID: 5300

UniProt: Q13526