LOX antibody (Middle Region)

Catalog No. ABIN3042506

The Rabbit Polyclonal anti-LOX antibody has been validated for WB and ELISA. It is suitable to detect LOX in samples from Human, Mouse and Rat.

Quick Overview for LOX antibody (Middle Region) (ABIN3042506)

Target: LOX (Lysyl Oxidase (LOX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LOX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LOX Antibody

Binding Specificity: AA 240-268, Middle Region

Purpose: Anti-LOX Antibody Picoband®

Sequence: AEENCLASTA YRADVRDYDH RVLLRFPQR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-LOX Antibody Picoband® (ABIN3042506). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human LOX, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Hamalainen, E.-R., Jones, T. A., Sheer, D., Taskinen, K., Pihlajaniemi, T., Kivirikko, K. I. Molecular cloning of human lysyl oxidase and assignment of the gene to chromosome 5q23.3-31.2. Genomics 11: 508-516, 1991. 2. Kuivaniemi, H., Savolainen, E.-R., Kivirikko, K. I. Human placental lysyl oxidase: purification, partial characterization, and preparation of two specific antisera to the enzyme. J. Biol. Chem. 259: 6996-7002, 1984.  3. Li, W., Nellaiappan, K., Strassmaier, T., Graham, L., Thomas, K. M., Kagan, H. M. Localization and activity of lysyl oxidase within nuclei of fibrogenic cells. Proc. Nat. Acad. Sci. 94: 12817-12822, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LOX

Target: LOX (Lysyl Oxidase (LOX))

Alternative Name: LOX

Background:

Synonyms: Protein-lysine 6-oxidase;1.4.3.13;Lysyl oxidase;LOX;

Tissue Specificity: Heart, placenta, skeletal muscle, kidney, lung and pancreas. .

Background: Lysyl oxidase  (LOX), also known as protein-lysine 6-oxidase, is a protein that, in humans, is encoded by the LOX gene. The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. 

Molecular Weight: 31 kDa

Gene ID: 4015

UniProt: P28300

Pathways: SARS-CoV-2 Protein Interactome