IL-18 antibody (AA 37-194)

Catalog No. ABIN3042518

The Rabbit Polyclonal anti-IL-18 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect IL-18 in samples from Mouse and Rat. There are 3+ publications available.

Quick Overview for IL-18 antibody (AA 37-194) (ABIN3042518)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-IL-18 Antibody

Binding Specificity: AA 37-194

Purpose: Anti-IL18 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IL18 Antibody Picoband® (ABIN3042518). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat IL-18 recombinant protein (Position: H37-S194). Rat IL-18 shares 64% and 91% amino acid (aa) sequences identity with human and mouse IL-18, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL,1. Okamura, H., Tsutsui, H., Komatsu, T., Yutsudo, M., Hakura, A., Tanimoto, T., Torigoe, K., Okura, T., Nukada, Y., Hattori, K., Akita, K., Namba, M., Tanabe, F., Konishi, K., Fukuda, S., Kurimoto, M. Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378: 88-91, 1995. 2. Reddy, P., Teshima, T., Kukuruga, M., Ordemann, R., Liu, C., Lowler, K., Ferrara, J. L. M. Interleukin-18 regulates acute graft-versus-host disease by enhancing Fas-mediated donor T cell apoptosis. J. Exp. Med. 194: 1433-1440, 2001. 3. Mallat, Z., Corbaz, A., Scoazec, A., Besnard, S., Leseche, G., Chvatchko, Y., Tedgui, A. Expression of interleukin-18 in human atherosclerotic plaques and relation to plaque instability. Circulation 104: 1598-1603, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-IL-18 antibody (ABIN3042518)

Si, Zhang, Gao, Chen, Zhao, Cheng: "Effects of IFN-? on IL-18 Expression in Pregnant Rats and Pregnancy Outcomes." in: Asian-Australasian journal of animal sciences, Vol. 26, Issue 10, pp. 1399-405, (2014) (PubMed).

Wang, Zhang, Zhang, Xu, Fang: "Expression and localization of IL-18 in the hypothalamic-pituitary-ovarian axis of non-pregnant, pregnant, and abortive rats." in: Journal of reproductive immunology, Vol. 92, Issue 1-2, pp. 45-53, (2011) (PubMed).

Yang, Shen, Chen, Ge: "Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion." in: World journal of gastroenterology, Vol. 11, Issue 29, pp. 4524-9, (2005) (PubMed).

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18

Background:

Synonyms: Interleukin-18,IL-18,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,Il18,Igif,

Tissue Specificity: Nervous system.

Background: Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses. IL18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.

Sequence Similarities: Belongs to the CNTF family.

Molecular Weight: 22 kDa

Gene ID: 29197

UniProt: P97636

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome