UBE2I antibody (C-Term)

Catalog No. ABIN3042573

Product Details anti-UBE2I Antibody

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Binding Specificity: AA 108-122, C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for SUMO-conjugating enzyme UBC9(UBE2I) detection. Tested with WB in Human,Mouse,Rat.

Sequence: IKQILLGIQE LLNE

Cross-Reactivity (Details): Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for SUMO-conjugating enzyme UBC9(UBE2I) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: ubiquitin-conjugating enzyme E2I
Protein Name: SUMO-conjugating enzyme UBC9

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human UBE2I(108-122aa IKQILLGIQELLNE), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBE2I (UBE2I Products)

Synonyms: C358B7.1 antibody, P18 antibody, UBC9 antibody, ubc9 antibody, ube2ia antibody, zUbc9 antibody, 5830467E05Rik antibody, F830028O17Rik antibody, Ubce2i antibody, Ubce9 antibody, UbcE2A antibody, ubce9 antibody, T13J8.70 antibody, T13J8_70 antibody, UBIQUITIN-PROTEIN LIGASE antibody, ubiquitin conjugating enzyme 9 antibody, ubiquitin conjugating enzyme E2 I antibody, ubiquitin-conjugating enzyme E2Ib antibody, ubiquitin-conjugating enzyme E2L antibody, ubiquitin-conjugating enzyme E2I antibody, ubiquitin conjugating enzyme E2I S homeolog antibody, ubiquitin conjugating enzyme 9 antibody, SUMO-conjugating enzyme UBC9 antibody, UBE2I antibody, ube2ib antibody, UBE2L antibody, Ube2i antibody, ube2i.S antibody, UBC9 antibody, ubc-9 antibody, LOC108703134 antibody

Background: SUMO-conjugating enzyme UBC9(UBE2I), also called UBC9, is a protein that in humans is encoded by the UBE2I gene. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. It is mapped to 16p13.3. UBC9 could fully complement the mutant phenotype of a yeast ubc9 mutant strain. This gene may play a similar role via interaction with WT1, which is able to impose a block to cell cycle progression in eukaryotic cells. What's more, it could support the growth of yeast ubc9 temperature-sensitive mutants at nonpermissive temperatures, indicating that the gene is a functional homolog of yeast ubc9. UBC9 is specifically associated with FHIT, such as FHIT may be involved in cell cycle control through its interaction with UBC9.

Synonyms: C358B7.1 antibody|Homologous to yeast UBC9 antibody|p18 antibody|SUMO 1 conjugating enzyme antibody|SUMO 1 protein ligase antibody|SUMO conjugating enzyme UBC9 antibody|SUMO protein ligase antibody|SUMO-conjugating enzyme UBC9 antibody|SUMO-protein ligase antibody|SUMO1 conjugating enzyme antibody|SUMO1 protein ligase antibody|UBC 9 antibody|UBC9 antibody|UBC9 homolog antibody|UBC9_HUMAN antibody|UBCE 9 antibody|UBCE9 antibody|UBE 2I antibody|Ube2i antibody|Ubiquitin carrier protein 9 antibody|Ubiquitin carrier protein antibody|Ubiquitin carrier protein I antibody|Ubiquitin conjugating enzyme 9 antibody|Ubiquitin conjugating enzyme E2I(homologous to yeast UBC9) antibody|Ubiquitin conjugating enzyme E2I(UBC9 homolog, yeast) antibody|Ubiquitin conjugating enzyme E2I antibody|Ubiquitin conjugating enzyme UbcE2A antibody|Ubiquitin like protein SUMO 1 conjugating enzyme antibody|Ubiquitin like protein SUMO1 conjugating enzyme antibody|Ubiquitin protein ligase E2I antibody|Ubiquitin protein ligase I antibody|Ubiquitin-conjugating enzyme E2 I antibody|Ubiquitin-protein ligase I antibody

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway