CCL2 antibody (AA 24-99)

Catalog No. ABIN3042598

This Rabbit Polyclonal antibody specifically detects CCL2 in WB, IHC and ELISA. It exhibits reactivity toward Human and has been mentioned in 21+ publications.

Quick Overview for CCL2 antibody (AA 24-99) (ABIN3042598)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CCL2 Antibody

Binding Specificity: AA 24-99

Purpose: Anti-MCP1/CCL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MCP1/CCL2 Antibody Picoband® (ABIN3042598). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MCP-1 recombinant protein (Position: Q24-T99). Human MCP-1 shares 60.9% and 59.4% amino acid (aa) sequence identity with mouse and rat MCP-1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Furutani, Y., Nomura, H., Notake, M., Oyamada, Y., Fukui, T., Yamada, M., Larsen, C. G., Oppenheim, J. J., Matsushima, K. Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF). Biochem. Biophys. 2. Gosling, J., Slaymaker, S., Gu, L., Tseng, S., Zlot, C. H., Young, S. G., Rollins, B. J., Charo, I. F. MCP-1 deficiency reduces susceptibility to atherosclerosis in mice that overexpress human apolipoprotein B. J. Clin. Invest. 103: 773-778, 1999. 3. Gu, L., Okaka, Y., Clinton, S. K., Gerard, C., Sukhova, G. K., Libby, P., Rollins, B. J. Absence of monocyte chemoattractant protein-1 reduces atherosclerosis in low absorbance lipoprotein receptor-deficient mice.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CCL2 antibody (ABIN3042598)

Qian, Feng, Sun, Xiong, Ding, Han, Chen, Chen, Du, Wang: "Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model." in: BioMed research international, Vol. 2018, pp. 8973986, (2019) (PubMed).

Xie, Li, Pi, Wu, Zeng, Zuo, Zha: "[Down-regulation of p38 MAPK and collagen by 1, 25-(OH)2-VD3 in rat models of diabetic nephropathy]." in: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, Vol. 32, Issue 7, pp. 931-5, (2017) (PubMed).

Li, Lu, Sun, Zuo, Wang, Yan: "Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm." in: PLoS ONE, Vol. 12, Issue 4, pp. e0174821, (2017) (PubMed).

Wu, Chang, Ren, Hu, Li, Liu: "Bindarit reduces the incidence of acute aortic dissection complicated lung injury via modulating NF-κB pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 3, pp. 2613-2618, (2017) (PubMed).

Sun, Zhang, Zhao, Zhen, Huang, Wang, He, Liu, Xu, Yang, Qu, Ma, Zhang, Zhang, Hu: "Attenuation of atherosclerotic lesions in diabetic apolipoprotein E-deficient mice using gene silencing of macrophage migration inhibitory factor." in: Journal of cellular and molecular medicine, Vol. 19, Issue 4, pp. 836-49, (2016) (PubMed).

Huang, Li, Niu, Xiao, Pu, Zheng, Qian: "Dynamic expressions of monocyte chemo attractant protein-1 and CC chamomile receptor 2 after balloon injury and their effects in intimal proliferation." in: Biomedical engineering online, Vol. 14, pp. 55, (2016) (PubMed).

Sun, Fan, Wang, Lu, Zhang, Wu, Shan, Zheng: "Purple sweet potato color inhibits endothelial premature senescence by blocking the NLRP3 inflammasome." in: The Journal of nutritional biochemistry, Vol. 26, Issue 10, pp. 1029-40, (2015) (PubMed).

Peng, Mao, Fu, Feng, Xu: "Comparison of biomarkers in rat renal ischemia-reperfusion injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 5, pp. 7577-84, (2015) (PubMed).

Liu, Li, Qiu, He: "Expression and cellular distribution of TLR4, MyD88, and NF-?B in diabetic renal tubulointerstitial fibrosis, in vitro and in vivo." in: Diabetes research and clinical practice, Vol. 105, Issue 2, pp. 206-16, (2014) (PubMed).

Tian, Lv, Yang, Zhang, Yu, Zhu, Xiao, Zhu: "Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats." in: International journal of clinical and experimental pathology, Vol. 7, Issue 6, pp. 3028-37, (2014) (PubMed).

Zhang, Wang, Ma, Liu, Zhang, Zhu: "Effect of herba centellae on the expression of HGF and MCP-1." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 427-434, (2013) (PubMed).

Sun, Bu, Liu, Yin, Zhou, Li, Xiao: "Therapeutic effects of human amniotic fluid-derived stem cells on renal interstitial fibrosis in a murine model of unilateral ureteral obstruction." in: PLoS ONE, Vol. 8, Issue 5, pp. e65042, (2013) (PubMed).

Liu, Wang, Yang, Wang, Yang, Liu, Ye: "Calcium oxalate monohydrate crystals stimulate monocyte chemoattractant protein-1 and transforming growth factor ?1 expression in human renal epithelial cells." in: Molecular medicine reports, Vol. 5, Issue 5, pp. 1241-4, (2012) (PubMed).

Lu, Zhu, Liang, Cui, Liu, Wu, Liang, Liu, Jiang, Liao, Xie, Zhou, Wu, Yuan, Liao: "Apelin-APJ induces ICAM-1, VCAM-1 and MCP-1 expression via NF-?B/JNK signal pathway in human umbilical vein endothelial cells." in: Amino acids, Vol. 43, Issue 5, pp. 2125-36, (2012) (PubMed).

Zhang, Li, Li, Zhao, Su, Zhang, Zhang, Miao: "Lipopolysaccharide activated phosphatidylcholine-specific phospholipase C and induced IL-8 and MCP-1 production in vascular endothelial cells." in: Journal of cellular physiology, Vol. 226, Issue 6, pp. 1694-701, (2011) (PubMed).

Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).

Ji, Gao, Cai, Lu, Hu, Wang, Cheng, Gu, Wan, Zhang, Cao: "Immunological regulation of Chinese herb Guizhi Fuling Capsule on rat endometriosis model." in: Journal of ethnopharmacology, Vol. 134, Issue 3, pp. 624-9, (2011) (PubMed).

Li, Wang, Yang, Zhang, Han, Wang: "Fluoxetine inhibited extracellular matrix of pulmonary artery and inflammation of lungs in monocrotaline-treated rats." in: Acta pharmacologica Sinica, Vol. 32, Issue 2, pp. 217-22, (2011) (PubMed).

He, Liang, Hou, Guo, Xie, Zhang: "Ethanol extraction of Picrorhiza scrophulariiflora prevents renal injury in experimental diabetes via anti-inflammation action." in: The Journal of endocrinology, Vol. 200, Issue 3, pp. 347-55, (2009) (PubMed).

Li, Hou, Zhang, Chen, Liu, Feng, Liu, Shan, Wang, Zhou, Tian, Xie: "Advanced oxidation protein products accelerate renal fibrosis in a remnant kidney model." in: Journal of the American Society of Nephrology : JASN, Vol. 18, Issue 2, pp. 528-38, (2007) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: CCL2

Background:

Synonyms: C-C motif chemokine 2,HC11,Monocyte chemoattractant protein 1,Monocyte chemotactic and activating factor,MCAF,Monocyte chemotactic protein 1,MCP-1,Monocyte secretory protein JE,Small-inducible cytokine A2,CCL2,MCP1, SCYA2,

Tissue Specificity: Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.

Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Sequence Similarities: Belongs to the intercrine beta (chemokine CC) family.

Molecular Weight: 11 kDa

Gene ID: 6347

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection