c-FOS antibody (Middle Region)

Catalog No. ABIN3042631

This Rabbit Polyclonal antibody specifically detects c-FOS in WB. It exhibits reactivity toward Human, Rat and Mouse. It has been mentioned in 12+ publications

Quick Overview for c-FOS antibody (Middle Region) (ABIN3042631)

Target: c-FOS (c-Fos) (c-Fos)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-FOS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-c-FOS Antibody

Binding Specificity: Middle Region

Purpose: Anti-c-Fos Antibody

Sequence: DQLEDEKSAL QTEIANLL

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-c-Fos Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human c-Fos, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-c-FOS antibody (ABIN3042631)

Zheng, Wu, Gao, Wang: "Tumor suppressive role of miR-569 in lung cancer." in: Oncology letters, Vol. 15, Issue 4, pp. 4087-4092, (2018) (PubMed).

Hou, Xia, Xiao, Wan, Zhao: "Neurotoxicity of intrathecal injections of dexmedetomidine into the rat spinal dorsal horn." in: Neural regeneration research, Vol. 7, Issue 23, pp. 1765-70, (2015) (PubMed).

Qiu, Ding, Cui, Hu, Ding: "The Expression Patterns of c-Fos and c-Jun Induced by Different Frequencies of Electroacupuncture in the Brain." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 343682, (2015) (PubMed).

Liu, Peng, Lin, Luo, Jiang, Mo, Yung: "Effect of rhynchophylline on the expression of p-CREB and sc-Fos in triatum and hippocampal CA1 area of methamphetamine-induced conditioned place preference rats." in: Fitoterapia, Vol. 92, pp. 16-22, (2014) (PubMed).

Zhang, Li, Xu, Mei, Zhang, Murong, Sun, Tong: "Changes of c-fos, malondialdehyde and lactate in brain tissue after global cerebral ischemia under different brain temperatures." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 354-8, (2014) (PubMed).

Zhang, Zhang, Goldstein, Li, Ge, He, Ruiz: "The effect of c-fos on acute myocardial infarction and the significance of metoprolol intervention in a rat model." in: Cell biochemistry and biophysics, Vol. 65, Issue 2, pp. 249-55, (2013) (PubMed).

Li, Liang, Chen, Zhang, Lin, Huang: "L-dopa methyl ester attenuates amblyopia-induced neuronal injury in visual cortex of amblyopic cat." in: Gene, Vol. 527, Issue 1, pp. 115-22, (2013) (PubMed).

Gong, Wang, Liang, Duan, Ouyang, Yu, Xu, Shen, Weng, Wu: "Soluble MOG35-55/I-A(b) dimers ameliorate experimental autoimmune encephalomyelitis by reducing encephalitogenic T cells." in: PLoS ONE, Vol. 7, Issue 10, pp. e47435, (2013) (PubMed).

Peng, Zhang, Ren, Zhang, Wang: "Effect of ketamine administration on memory consolidation, p-CREB and c-fos expression in the hippocampal slices of minor rats." in: Molecular biology reports, Vol. 38, Issue 4, pp. 2401-7, (2011) (PubMed).

Zhang, He, Goldstein, Ge, Wang, Ruiz: "The expression and significance of proto-oncogene c-fos in viral myocarditis." in: Virology journal, Vol. 7, pp. 285, (2010) (PubMed).

Lou, Jiang, Li, Zhou, He, Lei, Li, Jia: "Herb medicine Gan-fu-kang attenuates liver injury in a rat fibrotic model." in: Journal of ethnopharmacology, Vol. 128, Issue 1, pp. 131-8, (2010) (PubMed).

Xu, Tang, Li, Shi, Chen, Liang: "Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells." in: World journal of gastroenterology, Vol. 14, Issue 32, pp. 5008-14, (2008) (PubMed).

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: FOS

Background:

Background: The human oncogene c-fos is cellular homolog of the transforming gene of Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus which was mapped to a single human chromosome. c-Fos is encoded by the FOS gene. FOS was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. FOS is also a major component of the activator protein-1 (AP-1) transcription factor complex, which includes members of the JUN family. c-fos is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. Using transgenic and knockout mice, Grigoriadis et al. (1995) established a unique role for the proto-oncogene and nuclear transcription factor, Fos, in regulating the differentiation and activity of specific bone cell populations, both during normal development and in bone disease.

Gene Full Name: Fos proto-oncogene, AP-1 transcription factor subunit

Sequence Similarities: Belongs to the bZIP family. Fos subfamily.

Molecular Weight: 37 kDa

Gene ID: 2353

UniProt: P01100

Pathways: S100 Proteins