RIPK3 antibody (N-Term)

Catalog No. ABIN3042641

The Rabbit Polyclonal anti-RIPK3 antibody has been validated for WB. It is suitable to detect RIPK3 in samples from Human.

Quick Overview for RIPK3 antibody (N-Term) (ABIN3042641)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RIPK3 Antibody

Binding Specificity: AA 60-76, N-Term

Purpose: Anti-RIP3/RIPK3 Antibody Picoband®

Sequence: EVKAMASLDN EFVLRLE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RIP3/RIPK3 Antibody (ABIN3042641). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RIP3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Kaiser, W. J., Upton, J. W., Long, A. B., Livingston-Rosanoff, D., Daley-Bauer, L. P., Hakem, R., Caspary, T., Mocarski, E. S. RIP3 mediates the embryonic lethality of caspase-8-deficient mice. Nature 471: 368-372, 2011. 2. Sun, L., Wang, H., Wang, Z., He, S., Chen, S., Liao, D., Wang, L., Yan, J., Liu, W., Lei, X., Wang, X. Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148: 213-227, 2012. 3. Zhang, D.-W., Shao, J., Lin, J., Zhang, N., Lu, B.-J., Lin, S.-C., Dong, M.-Q., Han, J. RIP3, an energy metabolism regulator that switches TNF-induced cell death from apoptosis to necrosis. Science 325: 332-336, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: RIPK3

Background:

Synonyms: Receptor-interacting serine/threonine-protein kinase 3,2.7.11.1,RIP-like protein kinase 3,Receptor-interacting protein 3,RIP-3,RIPK3,RIP3,

Tissue Specificity: Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. .

Background: Receptor-interacting serine/threonine-protein kinase 3 (RIPK3), also known as RIP3 is an enzyme that in humans is encoded by the RIPK3 gene. This gene is mapped to 14q12. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Molecular Weight: 56 kDa, 77 kDa

UniProt: Q9Y572

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades