RIPK3 antibody (C-Term)

Catalog No. ABIN392275

Product Details anti-RIPK3 Antibody

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Binding Specificity: AA 489-518, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-518 amino acids from the C-terminal region of human RIPK3.

Clone: RB3319

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-RIPK3 antibody (ABIN392275)

McCullough, McMullen, Das, Roychowdhury, Strainic, Medof, Nagy: "Differential contribution of complement receptor C5aR in myeloid and non-myeloid cells in chronic ethanol-induced liver injury in mice." in: Molecular immunology, Vol. 75, pp. 122-32, (2017) (PubMed).

Deutsch, Graffeo, Rokosh, Pansari, Ochi, Levie, Van Heerden, Tippens, Greco, Barilla, Tomkötter, Zambirinis, Avanzi, Gulati, Pachter, Torres-Hernandez, Eisenthal, Daley, Miller: "Divergent effects of RIP1 or RIP3 blockade in murine models of acute liver injury." in: Cell death & disease, Vol. 6, pp. e1759, (2015) (PubMed).

Dara, Johnson, Suda, Win, Gaarde, Han, Kaplowitz: "Receptor interacting protein kinase 1 mediates murine acetaminophen toxicity independent of the necrosome and not through necroptosis." in: Hepatology (Baltimore, Md.), (2015) (PubMed).

Roychowdhury, Chiang, Mandal, McMullen, Liu, Cohen, Pollard, Feldstein, Nagy: "Inhibition of apoptosis protects mice from ethanol-mediated acceleration of early markers of CCl4 -induced fibrosis but not steatosis or inflammation." in: Alcoholism, clinical and experimental research, Vol. 36, Issue 7, pp. 1139-47, (2012) (PubMed).

Zorde-Khvalevsky, Abramovitch, Barash, Spivak-Pohis, Rivkin, Rachmilewitz, Galun, Giladi: "Toll-like receptor 3 signaling attenuates liver regeneration." in: Hepatology (Baltimore, Md.), Vol. 50, Issue 1, pp. 198-206, (2009) (PubMed).

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: RIPK3 (RIPK3 Products)

Background: RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Molecular Weight: 56887

Gene ID: 11035

NCBI Accession: NP_006862

UniProt: Q9Y572

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades