EPH Receptor B3 antibody (C-Term)

Catalog No. ABIN3042663

This anti-EPH Receptor B3 antibody is a Rabbit Polyclonal antibody detecting EPH Receptor B3 in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for EPH Receptor B3 antibody (C-Term) (ABIN3042663)

Target: EPH Receptor B3 (EPHB3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EPH Receptor B3 Antibody

Binding Specificity: AA 982-998, C-Term

Purpose: Anti-Eph receptor B3/EPHB3 Antibody Picoband®

Sequence: SIQDMRLQMN QTLPVQV

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Eph receptor B3/EPHB3 Antibody (ABIN3042663). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor B3, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Bohme, B., Holtrich, U., Wolf, G., Luzius, H., Grzeschik, K.-H., Strebhardt, K., Rubsamen-Waigmann, H.PCR mediated detection of a new human receptor-tyrosine-kinase, HEK 2.Oncogene 8: 2857-2862, 1993. 2. Bohme, B., VandenBos, T., Cerretti, D. P., Park, L. S., Holtrich, U., Rubsamen-Waigmann, H., Strebhardt, K.Cell-cell adhesion mediated by binding of membrane-anchored ligand LERK-2 to the EPH-related receptor human embryonal kinase 2 promotes tyrosine kinase activity.J. Biol. Chem. 271: 24747-24752, 1996.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EPH Receptor B3

Target: EPH Receptor B3 (EPHB3)

Alternative Name: EPHB3

Background:

Synonyms: Ephrin type-B receptor 3,2.7.10.1,EPH-like tyrosine kinase 2,EPH-like kinase 2,Embryonic kinase 2,EK2,hEK2,Tyrosine-protein kinase TYRO6,EPHB3,ETK2, HEK2, TYRO6,

Tissue Specificity: Ubiquitous.

Background: Ephrin Receptor EphB3, is also known as human embryo kinase2 (HEK2) or Eph-like tyrosine kinase2 (ETK2). HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases (LERKs), namely, LERK2 (EFNB1) and LERK5 (EFNB2). Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Molecular Weight: 130 kDa

UniProt: P54753

Pathways: RTK Signaling