LIF antibody (C-Term)

Catalog No. ABIN3042667

This Rabbit Polyclonal antibody specifically detects LIF in WB. It exhibits reactivity toward Mouse and Rat and has been mentioned in 7+ publications.

Quick Overview for LIF antibody (C-Term) (ABIN3042667)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIF Antibody

Binding Specificity: AA 175-194, C-Term

Purpose: Anti-Leukemia inhibitory factor LIF Antibody Picoband®

Sequence: DKEAFQRKKL GCQLLGTYKQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Leukemia inhibitory factor LIF Antibody (ABIN3042667). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse LIF, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Bamberger, A.-M., Jenatschke, S., Schulte, H. M., Loning, T., Bamberger, C. M. Leukemia inhibitory factor (LIF) stimulates the human HLA-G promoter in JEG3 choriocarcinoma cells. J. Clin. Endocr. Metab. 85: 3932-3936, 2000. 2. Sutherland, G. R., Baker, E., Hyland, V. J., Callen, D. F., Stahl, J., Gough, N. M. The gene for human leukemia inhibitory factor (LIF) maps to 22q12. Leukemia 3: 9-13, 1989.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-LIF antibody (ABIN3042667)

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." in: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, (2017) (PubMed).

Dong, Sui, Huang, Wang, Hu, Xiong, Wang, Zhang: "MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice." in: American journal of translational research, Vol. 8, Issue 2, pp. 1155-63, (2016) (PubMed).

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, (2012) (PubMed).

Xu, Sun, Li, Wu, Zhang, Feng: "Pinopodes, leukemia inhibitory factor, integrin-?3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss." in: Fertility and sterility, Vol. 98, Issue 2, pp. 389-95, (2012) (PubMed).

Li, Xu, Chen, Sun: "Effects of hydrosalpinx on pinopodes, leukaemia inhibitory factor, integrin beta3 and MUC1 expression in the peri-implantation endometrium." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 151, Issue 2, pp. 171-5, (2010) (PubMed).

Pei, Yu, Shi, Jia: "The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women." in: Contraception, Vol. 82, Issue 4, pp. 379-84, (2010) (PubMed).

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." in: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, (2007) (PubMed).

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background:

Synonyms: Leukemia inhibitory factor,LIF,Differentiation-stimulating factor,D factor,Lif,

Tissue Specificity: Not abundant, expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord. .

Background: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.

Sequence Similarities: Belongs to the tumor necrosis factor family.

Molecular Weight: 40 kDa

UniProt: P09056

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding