CDK1 antibody (C-Term)

Catalog No. ABIN3042681

This anti-CDK1 antibody is a Rabbit Polyclonal antibody detecting CDK1 in WB. Suitable for Human. This Primary Antibody has been cited in 10+ publications.

Quick Overview for CDK1 antibody (C-Term) (ABIN3042681)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDK1 Antibody

Binding Specificity: AA 279-297, C-Term

Purpose: Anti-Cyclin-dependent kinase 1 CDK1 Antibody Picoband®

Sequence: KMALNHPYFN DLDNQIKKM

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cyclin-dependent kinase 1 CDK1 Antibody (ABIN3042681). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CDK1, different from the relative rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Draetta, G., Piwnica-Worms, H., Morrison, D., Druker, B., Roberts, T., Beach, D. : Human CDC2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate. Nature 336: 738-744, 1988. 2. Spurr, N. K., Goodfellow, P. N., Nurse, P., Lee, M. : Assignment of the human homologue of the yeast cell cycle control gene CDC2 to chromosome 10. (Abstract) Cytogenet. Cell Genet. 46: 698, 1987. 3. Nazarenko, S. A., Ostroverhova, N. V., Spurr, N. K. : Regional assignment of the human cell cycle control gene CDC2 to chromosome 10q21 by in situ hybridization. Hum. Genet. 87: 621-622, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CDK1 antibody (ABIN3042681)

Zhang, Yao, Luan, Zhao, Jing, Zhang, Hou, Zou: "APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica." in: Scientific reports, Vol. 6, pp. 39047, (2018) (PubMed).

Lin, Zhu, Sun, Yang, Sun, Li, Cui, Zheng, Yu: "Prostate cancer cell proliferation is suppressed by microRNA-3160-5p via targeting of F-box and WD repeat domain containing 8." in: Oncology letters, Vol. 15, Issue 6, pp. 9436-9442, (2018) (PubMed).

Zhao, Zhao, Wang, Li, Zhang: "Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 64, Issue 2, pp. 125-37, (2016) (PubMed).

Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, (2015) (PubMed).

Zheng, Liu, Liu, Ma, Zhou, Chen, Chang, Wang, Yang, He: "Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH?SY5Y human neuroblastoma cells." in: Molecular medicine reports, Vol. 10, Issue 1, pp. 89-94, (2014) (PubMed).

Zeng, Xiang, Xue, Li, Ma, Ren, Xu, Jiao, Wang, Hu: "The cell death and DNA damages caused by the Tet-On regulating HSV-tk/GCV suicide gene system in MCF-7 cells." in: Biomedicine & pharmacotherapy = Biome?decine & pharmacothe?rapie, Vol. 68, Issue 7, pp. 887-92, (2014) (PubMed).

Yang, Yang, Sun, Yan, Meng, Ma: "Alantolactone inhibits growth of K562/adriamycin cells by downregulating Bcr/Abl and P-glycoprotein expression." in: IUBMB life, Vol. 65, Issue 5, pp. 435-44, (2013) (PubMed).

Lin, Fu, Zhao, Lin, Zou, Liu, Zhu, Wang, Yu: "Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells." in: Molecular biology reports, Vol. 38, Issue 3, pp. 1741-7, (2011) (PubMed).

Ye, Chen, Chen, Xie, Yang, Lou, Yu: "XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 133-41, (2009) (PubMed).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1

Background:

Synonyms: Cyclin-dependent kinase 1,CDK1,2.7.11.22,2.7.11.23,Cell division control protein 2 homolog,Cell division protein kinase 1,p34 protein kinase,CDK1,CDC2, CDC28A, CDKN1, P34CDC2,

Tissue Specificity: Isoform 2 is found in breast cancer tissues.

Background: CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Molecular Weight: 34 kDa

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA