Thrombospondin 2 antibody (Middle Region)

Catalog No. ABIN3042702

Product Details anti-Thrombospondin 2 Antibody

Target: Thrombospondin 2 (THBS2)

Binding Specificity: AA 637-655, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thrombospondin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Thrombospondin-2(THBS2) detection. Tested with WB, IHC-P in Human.

Sequence: EAAKTEKQVC EPENPCKDK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Thrombospondin-2(THBS2) detection. Tested with WB, IHC-P in Human.
Gene Name: thrombospondin 2
Protein Name: Thrombospondin-2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Thrombospondin 2(637-655aa EAAKTEKQVCEPENPCKDK), different from the mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Thrombospondin 2

Target: Thrombospondin 2 (THBS2)

Alternative Name: THBS2 (THBS2 Products)

Synonyms: THBS1 antibody, tsp2 antibody, thbs2 antibody, TSP2 antibody, TMEM200A antibody, TSP-2 antibody, Thbs-2 antibody, thrombospondin 2 antibody, thrombospondin 2 L homeolog antibody, thrombospondin 2a antibody, THBS2 antibody, thbs2.L antibody, thbs2a antibody, Thbs2 antibody

Background: Thrombospondin-2(THBS2) is a protein that in humans is encoded by the THBS2 gene. The protein encoded by this gene belongs to the thrombospondin family. The THBS2 is mapped to 6q27 and it is located on chromosome 17. The gene was transcribed in fibroblasts, smooth muscle cells, and an osteosarcoma cell line. It functions as a protein inhibitor of tumor growth and angiogenesis and modulates the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.

Synonyms: THBS2 antibody|Thrombospondin-2 antibody|TSP2 antibody|TSP2_HUMAN antibody

UniProt: P35442