NDRG1 antibody (Middle Region)

Catalog No. ABIN3042703

This Rabbit Polyclonal antibody specifically detects NDRG1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for NDRG1 antibody (Middle Region) (ABIN3042703)

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDRG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NDRG1 Antibody

Binding Specificity: AA 194-214, Middle Region

Purpose: Anti-Protein NDRG1 NDRG1 Antibody Picoband®

Sequence: HLFGKEEMQS NVEVVHTYRQ H

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Protein NDRG1 NDRG1 Antibody (ABIN3042703). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NDRG1.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. van Belzen N, Dinjens WN, Diesveld MP, Groen NA, van der Made AC, Nozawa Y, Vlietstra R, Trapman J, Bosman FT (Sep 1997). "A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms". Lab Invest 77 (1): 85-92. PMID 9251681. 2. Kokame K, Kato H, Miyata T (Jan 1997). "Homocysteine-respondent genes in vascular endothelial cells identified by differential display analysis. GRP78/BiP and novel genes". J Biol Chem 271 (47): 29659-65. 3. Zhang J, Chen S, Zhang W, Zhang J, Liu X, Shi H, Che H, Wang W, Li F, Yao L (Jun 2008). "Human differentiation-related gene NDRG1 is a Myc downstream-regulated gene that is repressed by Myc on the core promoter region". Gene 417 (1-2): 5-12. 4. "Entrez Gene: NDRG1 N-myc downstream regulated gene 1".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NDRG1

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Alternative Name: NDRG1

Background:

Synonyms: Protein NDRG1,Differentiation-related gene 1 protein,DRG-1,N-myc downstream-regulated gene 1 protein,Nickel-specific induction protein Cap43,Reducing agents and tunicamycin-responsive protein,RTP,Rit42,NDRG1,CAP43, DRG1, RTP,

Tissue Specificity: Ubiquitous, expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression. .

Background: Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. It is a member of the N-myc downregulated gene family which belong to the alpha/beta hydrolase superfamily. The NDRG1 gene is mapped to chromosome 8q24. It has got a deduced 394 amino acid protein with a molecular mass of 43kD. NDRG1 is expressed in the cytoplasm and basolateral membranes of gut lumen surface epithelial cells. The protein appears to play a vital role in growth arrest and cell differentiation.

Sequence Similarities: Belongs to the NDRG family.

Molecular Weight: 36 kDa

UniProt: Q92597