ICA1 antibody (Middle Region)

Catalog No. ABIN3042731

The Rabbit Polyclonal anti-ICA1 antibody is suitable to detect ICA1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for ICA1 antibody (Middle Region) (ABIN3042731)

Target: ICA1 (Islet Cell Autoantigen 1, 69kDa (ICA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ICA1 Antibody

Binding Specificity: AA 243-276, Middle Region

Purpose: Anti-ICA1 Antibody

Sequence: EKTSHTMAAI HESFKGYQPY EFTTLKSLQD PMKK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ICA1 Antibody (ABIN3042731). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ICA1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ICA1

Target: ICA1 (Islet Cell Autoantigen 1, 69kDa (ICA1))

Alternative Name: ICA1

Background:

Synonyms: Islet cell autoantigen 1,69 kDa islet cell autoantigen,ICA69,Islet cell autoantigen p69,ICAp69,p69,ICA1,

Tissue Specificity: Expressed abundantly in pancreas, heart and brain with low levels of expression in lung, kidney, liver and thyroid.

Background: Islet cell autoantigen 1 is a protein that in humans is encoded by the ICA1 gene. It is mapped to 7p22. This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. What's more, this protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene.

Sequence Similarities: Belongs to the CTCF zinc-finger protein family.

Molecular Weight: 60-65 kDa

Gene ID: 3382

UniProt: Q05084