IL12B antibody (AA 23-321)

Catalog No. ABIN3042735

This anti-IL12B antibody is a Rabbit Polyclonal antibody detecting IL12B in WB. Suitable for Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for IL12B antibody (AA 23-321) (ABIN3042735)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL12B Antibody

Binding Specificity: AA 23-321

Purpose: Anti-IL12 p40/IL12B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IL12 p40/IL12B Antibody (ABIN3042735). Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat IL-12 (p40) recombinant protein (Position: M23-S321).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat
1. Entrez Gene: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) 2. Yokouchi, Y., Nukaga, Y., Shibasaki, M., Noguchi, E., Kimura, K., Ito, S., Nishihara, M., Yamakawa-Kobayashi, K., Takeda, K., Imoto, N., Ichikawa, K., Matsui, A., Hamaguchi, H., Arinami, T. Significant evidence for linkage of mite-sensitive childhood asthma to chromosome 5q31-q33 near the interleukin 12 B locus by a genome-wide search in Japanese families. Genomics 66: 152-160, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-IL12B antibody (ABIN3042735)

Dong, Xia, Chen, Chen, Zhang: "Anti-arthritis activity of cationic materials." in: Journal of cellular and molecular medicine, Vol. 14, Issue 7, pp. 2015-24, (2011) (PubMed).

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL12B

Background:

Synonyms: Interleukin-12 subunit beta ,Cytotoxic lymphocyte maturation factor 40 kDa subunit ,IL-12 subunit p40 ,Il12b ,IL12B ,

Background: Subunit beta of interleukin 12, also known as interleukin 12B, or IL12B, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.

Sequence Similarities: Belongs to the type I cytokine receptor family. Type 3 subfamily.

Molecular Weight: 20 kDa

Gene ID: 64546

UniProt: Q9R278

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation